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Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA Genet. Mol. Biol.
Freire,Maíra C. M.; Oliveira,Luiz O. de; Almeida,Álvaro M. R. de; Schuster,Ivan; Moreira,Maurilio A.; Liebenberg,Merion M.; Mienie,Charlotte M. S..
Phakopsora pachyrhizi has dispersed globally and brought severe economic losses to soybean growers. The fungus has been established in Brazil since 2002 and is found nationwide. To gather information on the temporal and spatial patterns of genetic variation in P. pachyrhizi , we sequenced the nuclear internal transcribed spacer regions (ITS1 and ITS2). Total genomic DNA was extracted using either lyophilized urediniospores or lesions removed from infected leaves sampled from 26 soybean fields in Brazil and one field in South Africa. Cloning prior to sequencing was necessary because direct sequencing of PCR amplicons gave partially unreadable electrophoretograms with peak displacements suggestive of multiple sequences with length polymorphism. Sequences...
Palavras-chave: Phakopsora pachyrhizi; Diversity; Internal transcribed spacer; Asian soybean rust; Phylogeography.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500020
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In vivo sensitivity of Phakopsora pachyrhizi to fungicides Ciência Rural
Chechi,Amanda; Ghissi-Mazetti,Valéria Cecília; Zuchelli,Elias; Deuner,Carolina Cardoso; Forcelini,Carlos Alberto; Boller,Walter.
ABSTRACT: Asian soybean rust is one of the most destructive diseases that can be found in this crop. It can be largely controlled by fungicide application. The objective was to assess the sensitivity of P. pachyrhizi isolates to fungicides. The tests were performed in a completely randomized design, with six replicates. The sensitivity of twelve isolates to site-specific and multisite fungicides at concentrations of 0.1; 1.0; 10.0, and 100.0 mg L-1, plus a control with absence of fungicide (0.0 mg L-1) was assessed. Soybean leaflets were immersed in the appropriate fungicide solutions, disposed in wet chambers in plastic boxes, and inoculated using each uredinia suspension of P. pachyhrizi (5.0 x 104 uredospores mL-1), separately. Boxes were incubated for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asian soybean rust; Detached leaf test; Effective concentration; Multisite fungicide; Soybean..
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000100151
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Inducers of resistance and silicon on the activity of defense enzymes in the soybean-Phakopsora pachyrhizi interaction Bragantia
Cruz,Maria Fernanda Antunes da; Rodrigues,Fabrício Ávila; Polanco,Leonora Rodriguez; Curvêlo,Carmen Rosa da Silva; Nascimento,Kelly Juliane Telles; Moreira,Maurílio Alves; Barros,Everaldo Gonçalves.
This study aimed to determine the effect of jasmonic acid (JA), Acibenzolar-S-Methyl (ASM) and calcium silicate (a source of soluble silicon, Si), on the potentiation of soybean resistance to Asian soybean rust (ASR). The ASR severity was significantly reduced on plants sprayed with ASM or supplied with Si in comparison to plants sprayed with JA or deionized water. For chitinases (CHI), significant differences in activity between non-inoculated and inoculated plants sprayed with deionized water or with ASM occurred at 72 hours after inoculation (hai), at 24 and 72 hai when sprayed with JA and at 141 hai when supplied with Si. For β-1,3-glucanases (GLU), significant differences in activity between non-inoculated and inoculated plants sprayed with deionized...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Asian soybean rust; Induction of resistance; Mechanisms of host defense.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052013000200008
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Molecular characterization of beta-tubulin from Phakopsora pachyrhizi, the causal agent of Asian soybean rust Genet. Mol. Biol.
Maciel,Talles Eduardo Ferreira; Freire,Maíra Cristina Menezes; Almeida,Álvaro M.R. de; Oliveira,Luiz Orlando de.
β-tubulins are structural components of microtubules and the targets of benzimidazole fungicides used to control many diseases of agricultural importance. Intron polymorphisms in the intron-rich genes of these proteins have been used in phylogeographic investigations of phytopathogenic fungi. In this work, we sequenced 2764 nucleotides of the β-tubulin gene (Pp tubB) in samples of Phakopsora pachyrhizi collected from seven soybean fields in Brazil. Pp tubB contained an open reading frame of 1341 nucleotides, including nine exons and eight introns. Exon length varied from 14 to 880 nucleotides, whereas intron length varied from 76 to 102 nucleotides. The presence of only four polymorphic sites limited the usefulness of Pp tubB for phylogeographic studies in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asian soybean rust; Beta-tubulin; Genetic diversity; Phakopsora pachyrhizi; Soybeans.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200025
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New insights into Phakopsora pachyrhizi infection based on transcriptome analysis in planta Genet. Mol. Biol.
Rincão,Michelle Pires; Carvalho,Mayra Costa da Cruz Gallo de; Nascimento,Leandro Costa; Lopes-Caitar,Valéria S.; Carvalho,Kenia de; Darben,Luana M.; Yokoyama,Alessandra; Carazzolle,Marcelo Falsarella; Abdelnoor,Ricardo Vilela; Marcelino-Guimarães,Francismar Correa.
Abstract Asian soybean rust (ASR) is one of the most destructive diseases affecting soybeans. The causative agent of ASR, the fungus Phakopsora pachyrhizi, presents characteristics that make it difficult to study in vitro, limiting our knowledge of plant-pathogen dynamics. Therefore, this work used leaf lesion laser microdissection associated with deep sequencing to determine the pathogen transcriptome during compatible and incompatible interactions with soybean. The 36,350 generated unisequences provided an overview of the main genes and biological pathways that were active in the fungus during the infection cycle. We also identified the most expressed transcripts, including sequences similar to other fungal virulence and signaling proteins. Enriched P....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asian soybean rust; Multigenic families; Transposable elements.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400671
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Simulating the U.S. Impacts of Alternative Asian Soybean Rust Treatment Regimes AgEcon
Johansson, Robert C.; Livingston, Michael J.; Westra, John V.; Guidry, Kurt M..
Asian soybean rust (rust) is an emerging issue in U.S. crop production and was identified in nine states during 2004. Recent farm surveys indicate that many producers are adjusting their management practices to the possibility of a rust infestation. The economic and environmental impacts of such adjustments are not known in the medium run given these new developments. We combine 2005 data on the geographical distribution of the fungal pathogen that causes rust with 2005 information on the availability and material costs of fungicides to analyze three treatment strategies. Our results indicate a higher range of economic impacts than previous research has indicated, but are consistent with earlier findings indicating that rust infestations will likely...
Tipo: Journal Article Palavras-chave: Asian soybean rust; Invasive species; Phakopsora pachyrhizi; Preventative and curative fungicides; Crop Production/Industries.
Ano: 2006 URL: http://purl.umn.edu/10186
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U.S. Soybean Producer Perceptions and Management of Soybean Rust in the United States under the USDA Pest Information Platform for Extension and Education AgEcon
Livingston, Michael J..
Recent survey data are examined to improve current understanding of the factors that help to determine the value of information reported on a website that serves as the centerpiece of the USDA’s “Soybean Rust Integrated Pest Management—Pest Information Platform for Extension and Education.” Respondents’ initial beliefs about their chances of experiencing a rust outbreak are shown to affect the likelihood that soybean producers will visit the website and change their management of fungicide use as a result.
Tipo: Journal Article Palavras-chave: Asian soybean rust; Probability beliefs; PIPE website; ARMS data; USDA Rural Development Broadband Program; Community/Rural/Urban Development; Crop Production/Industries; Risk and Uncertainty.
Ano: 2010 URL: http://purl.umn.edu/95647
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Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi Electron. J. Biotechnol.
Lourdes Rocha,Carla Maria; Vellicce,Gabriel Ricardo; García,María Gabriela; Pardo,Esteban Mariano; Racedo,Josefina; Perera,María Francisca; de Lucía,Adrian; Gilli,Javier; Bogado,Noelia; Bonnecarrère,Victoria; German,Silvia; Marcelino,Francismar; Ledesma,Fernando; Reznikov,Sebastián; Ploper,Leonardo Daniel; Welin,Bjorn; Castagnaro,Atilio Pedro.
Background Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of...
Tipo: Journal article Palavras-chave: Asian soybean rust; Genetic variation; Glycine max; Molecular markers.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600009
Registros recuperados: 8
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